Three-dimensional (3D) cell culture models have emerged over the past 15 years and have become a key reference in the field of cell culture, as they better replicate the functions and architecture of tissues in a physiologically relevant way. They are used both in fundamental research, for example to understand organogenesis, and in pharmacology for the development of new therapeutic compounds.
Organoids: 3D Cellular Models That Mimic Mini-Organs
Today, numerous techniques exist for assembling cells in 3D to generate structures known as organoids, which are, by definition, cell aggregates that mimic the properties of a mini-organ (such as kidney, brain, intestine, etc.).
Building Organoids: From Patient Cells to Self-Assembled Mini-Organs
Choose a successful formation of a relevant organoid depends on several factors, including the origin of the cells used such as embryonic stem cells, induced pluripotent stem cells, or primary cells and the technology used to produce them, with an increasing number of models relying on the cells' remarkable self-assembly capabilities. In most cases, the cells are derived from patient biopsies, usually from epithelial tissues, obtained through mechanical and/or enzymatic dissociation of the tissue, followed by cell purification to remove the extracellular matrix (e.g., collagen, laminin) and stromal cells (such as immune cells and fibroblasts).brant image and write an inspiring paragraph about it. It does not have to be long, but it should reinforce your image.